In order to comprehensively reflect the quantity, quality and efficiency of regional water resource development and utilization, a framework of absolute balance sheet of water resource was proposed.The concepts and the associated determination methods for resource coefficient, quality coefficient and efficiency coefficient were proposed, and the quantification methods of the absolute assets and liabilities of water resource were given. Taking Jinan City of Shandong Province as an example, based on the proposed method, the resource coefficient, quality coefficient and efficiency coefficient, which could reflect the quantity, quality and utilization efficiency of local water resource, were calculated. The calculated absolute assets from 2011 to 2015 were 0.28×108, 0.37×108, 0.54×108, 0.26×108 and 0.25×108 m3 respectively, and the absolute liabilities were 0.64×108, 0.42×108, 0.71×108, 0.19×108 and 0.64×108 m3 respectively. This research could be considered as a further in-depth innovation on the existing relative balance sheet of water resource, and had theoretical significance and practical values on the establishment of absolute balance sheet and regional sustainable development of water resource.