一种胶原支架材料的结构与性能表征

山东大学学报(工学版) ›› 2010, Vol. 40 ›› Issue (4): 67-71.

一种胶原支架材料的结构与性能表征

胡顺鹏1,赵洪石2,王冠聪2,曹成波1*,刘宏2*,李文波1,杨晓宇1

1. 山东大学化学与化工学院, 山东济南 250100; 2. 山东大学晶体材料国家重点实验室, 山东济南 250100

收稿日期:2010-02-09 出版日期:2010-08-16 发布日期:2010-02-09
通讯作者: 曹成波(1965-),男,山东招远人,教授,博士,主要研究方向为生物医用高分子材料、组织工程、有机精细化学工程. E-mail:E-mail: cbcao@sdu.edu.cn
作者简介:胡顺鹏(1985-),男,山东临沂人,硕士研究生,主要研究方向为组织工程材料.E-mail: hughboo@yahoo.com.cn
基金资助:
国家自然科学基金杰出青年基金资助项目(50925205);山东省重点科技攻关资助项目(2008GG10002031)

Structure and properties of a collagen scaffold material

HU Shun-peng1, ZHAO Hong-shi2, WANG Guan-cong2, CAO Cheng-bo1*, LIU Hong2*, LI Wen-bo1, YANG Xiao-yu1

1. School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;
2. State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China

Received:2010-02-09 Online:2010-08-16 Published:2010-02-09

摘要/Abstract

摘要：

针对脱细胞真皮基质(acellular dermal matrix, ADM)孔径小、孔隙率低、孔隙的连通性不理想的难题,以猪中厚皮为原料,综合碱处理、酶处理、去污剂处理和盐处理手段制备了一种支架材料,通过H＆E染色、红外与紫外可见光谱、扫描电子显微镜、拉力试验、MTT法等手段分析了支架材料的性能。结果显示:支架材料维持了胶原的天然结构,不含细胞成分,紫外与红外吸收符合胶原蛋白的吸收特征,胶原质量分数为(83.19±0.24)％,为具有连通孔道的三维网络结构,孔径约100μm,孔隙率为(76.94±1.08)％,极限抗拉强度为(4.43±0.10)MPa,拉伸弹性模量为(36.08±1.52)MPa,小鼠L929成纤维细胞在其上增殖良好。

关键词: 支架材料, 胶原, 孔结构, 力学性能, 细胞增殖

Abstract:

Collagen matrix acellular dermal matrix (ADM) has been widely used as scaffold material in skin tissue engineering. However, its pore diameter is small, its porosity is low, and its pore interconnectivity is poor. In light of this, a collagen scaffold material with suitable pore diameter, high porosity and obvious interconnected pore structure was prepared by means of the combined treatments of enzyme, alkali, sodium dodecyl sulfonate and sodium chloride solution, and its structure and properties were analyzed. UV and FTIR spectra showed the typical collagen peaks, and the content of collagen was (83.19±0.24) ％. Scanning electron microscopy (SEM) images displayed that the scaffold material had threedimensional interconnected channels with a diameter of about 100μm. The porosity of the scaffold material was (76.94±1.08)％, the ultimate tensile strength was (4.43±0.10) MPa, and the elastic modulus was (36.08±1.52) MPa. Mouse fibroblasts L929 were cultured on the scaffold material, and cell proliferation tests were performed by MTT assay. Results from the MTT assay indicated that the cells cultured on the scaffold material could proliferate well.

Key words: scaffold material, collagen, pore structure, mechanical property, cell proliferation

胡顺鹏1,赵洪石2,王冠聪2,曹成波1*,刘宏2*,李文波1,杨晓宇1. 一种胶原支架材料的结构与性能表征[J]. 山东大学学报(工学版), 2010, 40(4): 67-71.

HU Shun-peng1, ZHAO Hong-shi2, WANG Guan-cong2, CAO Cheng-bo1*, LIU Hong2*, LI Wen-bo1, YANG Xiao-yu1. Structure and properties of a collagen scaffold material[J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2010, 40(4): 67-71.

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