Collagen matrix acellular dermal matrix (ADM) has been widely used as scaffold material in skin tissue engineering. However, its pore diameter is small, its porosity is low, and its pore interconnectivity is poor. In light of this, a collagen scaffold material with suitable pore diameter, high porosity and obvious interconnected pore structure was prepared by means of the combined treatments of enzyme, alkali, sodium dodecyl sulfonate and sodium chloride solution, and its structure and properties were analyzed. UV and FTIR spectra showed the typical collagen peaks, and the content of collagen was (83.19±0.24) ％. Scanning electron microscopy (SEM) images displayed that the scaffold material had threedimensional interconnected channels with a diameter of about 100μm. The porosity of the scaffold material was (76.94±1.08)％, the ultimate tensile strength was (4.43±0.10) MPa, and the elastic modulus was (36.08±1.52) MPa. Mouse fibroblasts L929 were cultured on the scaffold material, and cell proliferation tests were performed by MTT assay. Results from the MTT assay indicated that the cells cultured on the scaffold material could proliferate well.