JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE) ›› 2017, Vol. 47 ›› Issue (4): 124-130.doi: 10.6040/j.issn.1672-3961.0.2017.050

Previous Articles    

Performance of mature aerobic granules under the absence of organic carbon source

YUAN Shasha, YU Haibo, GAO Mingming, WANG Xinhua*   

  1. School of Environmental Science &
    Engineering, Shandong University, Jinan 250100, Shandong, China
  • Received:2017-02-15 Online:2017-08-20 Published:2017-02-15

Abstract: Physicochemical properties, microbial activities, extracellular polymeric substances(EPS)and structure stability of mature aerobic granules were studied to investigate the effect of organic carbon source absence on the stability and nitrification activity of aerobic granules, the capability of upholding shock of aerobic granules was tested. Results showed that microbial activity of ammonia oxidizing bacteria(AOB)and nitrite oxidizing bacteria(NOB)increased, and high nitrifying activity achieved on 60 d under the absence of organic carbon source. Under the absence of organic carbon source, particle size of aerobic granules decreased. Aerobic granules became compacter with loss of filamentous bacteria and increase of spherical bacteria. EPS mass concentration and the mass ratio of polysaccharide and proteins increased. Under the absence of organic carbon source, mature aerobic granules didnt disintegrate completely and had good capability of upholding shock. This study verified the potential of aerobic granules treating wastewater under the absence of organic carbon source, and provided fundamental basis for the practical application of aerobic granules.

Key words: aerobic granules, organic carbon source, extracellular polymeric substance, microbial activity, physicochemical properties

CLC Number: 

  • X703.1
[1] MU Y, YU H Q. Biological hydrogen production in a UASB reactor with granules. I: physicochemical characteristics of hydrogen-producing granules[J]. Biotechnology and Bioengineering, 2006, 94(5):980-987.
[2] ADAV S S, CHEN M Y, LEE D J, et al. Degradation of phenol by aerobic granules and isolated yeast Candida tropicalis[J]. Biotechnology and Bioengineering, 2007, 96(5):844-852.
[3] 李善评,赵玉晓, 乔鹏, 等.好氧颗粒污泥的培养及基质降解和污泥生长动力学分析[J]. 山东大学学报(工学版), 2008, 38(3):95-98. LI Shanping, ZHAO Yuxiao, QIAO Peng, Cultivation of aerobic granular sludge and the kinetics of substrate degradation and biomass growth[J]. Journal of Shandong University(Engineering Science), 2008, 38(3):95-98.
[4] PRONK M, DE KREUK M K, DE BRUIN B, et al. Full scale performance of the aerobic granular sludge process for sewage treatment[J]. Water Research, 2015, 84:207-217.
[5] LIU Y Q, TAY J H. Fast formation of aerobic granules by combining strong hydraulic selection pressure with over stressed organic loading rate[J]. Water Research, 2015, 80:256-266.
[6] LIU Y Q, KONG Y, TAY J H, et al. Enhancement of start-up of pilot-scale granular SBR fed with real wastewater[J]. Separation and Purification Technology, 2011, 82(1):190-196.
[7] YANG F L, WANG X H, ZHANG H M, et al. A review on the essential role of substrate on aerobic granulation[J]. International Journal of Environment and Waste Management, 2011, 7(1/2):67-79.
[8] KIM D, KIM T S, RYU H D, et al. Treatment of low carbon-to-nitrogen wastewater using two-stage sequencing batch reactor with independent nitrification[J]. Process Biochemistry, 2008, 43(4):406-413.
[9] LUO J, HAO T, WEI L, et al. Impact of influent COD/N ratio on disintegration of aerobic granular sludge[J]. Water Research, 2014, 62(7):127-135.
[10] WANG X H, ZHANG H M, YANG F L, et al. Improved stability and performance of aerobic granules under stepwise increased selection pressure[J]. Enzyme and Microbial Technology, 2007, 41(3):205-211.
[11] LIU Y, YANG S F, TAY J H. Elemental compositions and characteristics of aerobic granules cultivated at different substrate N/C ratios[J]. Applied Microbiology and Biotechnology, 2003, 61(5):556-561.
[12] YANG S F, TAY J H, LIU Y. Respirometric activities of heterotrophic and nitrifying populations in aerobic granules developed at different substrate N/COD ratios[J]. Current Microbiology, 2004, 49(1):42-46.
[13] CARUCCI A, CHIAVOLA A, MAJONE M, et al. Treatment of tannery wastewater in a sequencing batch reactor[J]. Water Science and Technology, 1999, 40(1):253-259.
[14] ZHANG B, CHEN Z, QIU Z G, et al. Dynamic and distribution of ammonia-oxidizing bacteria communities during sludge granulation in an anaerobic-aerobic sequencing batch reactor[J]. Water Research, 2011, 45(18):6207-6216.
[15] FROLUND B, PALMGREN R, KEIDING K, et al. Extraction of extracellular polymers from activated sludge using a cation exchange resin[J]. Water Research, 1996, 30(8):1749-1758.
[16] LOEWUS F A. Improvement in anthrone method for determination of carbohydrates[J]. Analytical Chemistry, 1952, 24(1):219-219.
[17] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1):248-254.
[18] HOU X L, LIU S T, ZHANG Z T. Role of extracellular polymeric substance in determining the high aggregation ability of anammox sludge[J]. Water Research, 2015, 75:51-62.
[19] RUIJSSENAARS H J, STINGELE F, HARTMANS S. Biodegradability of food-associated extracellular polysaccharides[J]. Current Microbiology, 2000, 40(3):194-199.
[20] WANG Z W, LIU Y, TAY J H. Biodegradability of extracellular polymeric substances produced by aerobic granules[J]. Applied Microbiology and Biotechnology, 2007, 74(2):462-466.
[21] ZHANG X Q, BISHOP P L. Biodegradability of biofilm extracellular polymeric substances[J]. Chemosphere, 2003, 50(1):63-69.
[22] JANG A, YOON Y H, KIM I S, et al. Characterization and evaluation of aerobic granules in sequencing batch reactor[J]. Journal of Biotechnology, 2003, 105(1-2):71-82.
[23] MATSUMOTO S, KATOKU M, SAEKI G, et al. Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses[J]. Environ Microbiol, 2010, 12(1):192-206.
[24] BASSIN J P, KLEEREBEZEM R, ROSADO A S, et al. Effect of different operational conditions on biofilm development, nitrification, and nitrifying microbial population in moving-bed biofilm reactors[J]. Environmental Science & Technology, 2012, 46(3):1546-1555.
[25] LIU Y, LIU Q S. Causes and control of filamentous growth in aerobic granular sludge sequencing batch reactors[J]. Biotechnology Advances, 2006, 24(1):115-127.
[26] SHENG G P, YU H Q, LI X Y. Extracellular polymeric substances(EPS)of microbial aggregates in biological wastewater treatment systems:a review[J]. Biotechnology Advances, 2010, 28(6):882-894.
[27] 王永飞, 张捍民, 王新华, 等. 曝气量对SBAR中好氧颗粒污泥特性的影响[J]. 环境科学, 2008, 29(6):1598-1603. WANG Yongfei, ZHANG Hanmin, WANG Xinhua, et al. Effects of aeration intensity on characteristics of aerobic granules in sequencing batch airlift reactor(SBAR)[J]. Environmental Science, 2008, 29(6):1598-1603.
[28] 刘燕,王越兴,莫华娟,等. 有机底物对活性污泥胞外聚合物的影响[J]. 环境化学, 2004, 23(3):252-257. LIU Yan, WANG Yuexing, MO Huajuan, et al. Effect of organic substrate on the formation of extracellular polymeric substrates in activated sludge[J]. Environmental Chemistry, 2004, 23(3):252-257.
[29] ZHU L, LYU M L, DAI X, et al. Role and significance of extracellular polymeric substances on the property of aerobic granule[J]. Bioresource Technology, 2012, 107(2):46-54.
[30] ZHANG H M, HE Y L, JIANG T, et al. Research on characteristics of aerobic granules treating petrochemical wastewater by acclimation and co-metabolism methods[J]. Desalination, 2011, 279(1-3):69-74.
[31] ADAV S S, LEE D J, TAY J H. Extracellular polymeric substances and structural stability of aerobic granule[J]. Water Research, 2008, 42(6-7):1644-1650.
[32] KOCATURK I, ERGUDER T H. Influent COD/TAN ratio affects the carbon and nitrogen removal efficiency and stability of aerobic granules[J]. Ecological Engineering, 2016, 90:12-24.
[33] 张丽丽, 陈效, 陈建孟, 等. 胞外多聚物在好氧颗粒污泥形成中的作用机制[J]. 环境科学, 2007, 28(4):795-799. ZHANG Lili, CHEN Xiao, CHEN Jianmeng, et al. Role mechanism of extracellular polymeric substances in the formation of aerobic granular sludge[J]. Environmental Science, 2007, 28(4):795-799.
[34] 王浩宇, 苏本生, 黄丹, 等. 好氧污泥颗粒化过程中Zeta 电位与EPS 的变化特性[J]. 环境科学, 2012, 33(5):1614-1620. WANG Haoyu,SU Bensheng, HUANG Dan, et al. Profiles of zeta potential and EPS in granulation process of aerobic sludge[J]. Environmental Science, 2012, 33(5):1614-1620.
[1] LI Fazhan, ZHANG Shuai, ZHANG Jian, LIANG Shuang. Study on C-CBR integrated bioreactor for treatment of rural domestic sewage [J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2018, 48(1): 124-130.
[2] YU Meiqiong, YANG Jinbei, CHEN Xiuyu, DENG Shu. Adsorption of Pb(II) and Ni(II) by pine sawdust [J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2015, 45(2): 75-81.
[3] YUAN Ai-juan,YUE Qin-yan,LI Qian,GAO Bao-yu,LI Jing . Preparation of granulating modified-bentonites and its decoloration performances to dyes [J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2008, 38(5): 88-93 .
[4] LI Shan-ping,ZHAO Yu-xiao,QIAO Peng,FENG Zheng-zhi . Cultivation of aerobic granular sludge and the kinetics of substrate degradation and biomass growth [J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2008, 38(3): 95-98 .
[5] ZHAO Yu-xiao,LI Shan-ping,QIAO Peng,ZHANG Qi-lei . Effect of phosphorus removal and microbial morphology of a biologically aerated filter [J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2008, 38(1): 88-91 .
[6] LI Shan-ping,HU Zhen,SUN Yi-ming*,ZHEN Bo-ru,ZHANG Qi-lei,CAO Han-lin . Preparation and evaluation of the electro-catalytic characteristics of novel lead Ti-based dioxide electrodes [J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2007, 37(3): 109-113 .
[7] ZHENG Nan, LI Cong, XIE Huijun, ZHANG Jian. The mechanism of effect on N2O production of carbon source types in denitrifying phosphorus removal process [J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2014, 44(5): 72-77.
Full text



No Suggested Reading articles found!