D4020大孔树脂分离姜油树脂中的姜酚

山东大学学报(工学版) ›› 2011, Vol. 41 ›› Issue (3): 148-153.

D4020大孔树脂分离姜油树脂中的姜酚

王艳,杜爱玲*,薛岩明,杜爱琴

山东大学化学与化工学院, 山东济南 250061

收稿日期:2011-01-22 出版日期:2011-06-16 发布日期:2011-01-22
通讯作者: 杜爱玲(1956- ),女,山东烟台人,教授,硕士,主要研究方向为天然产物有效成分的提取分离以及材料腐蚀与防护. Email: duail@sina.com E-mail:duail@sina.com
作者简介:王艳(1986- ),女,山东枣庄人,硕士研究生,主要研究方向为天然产物有效成分的分离.Email: wangyan121314@126.com
基金资助:
科技部农业科技成果转化资助项目(2008GB2C600178)

Separation of gingerols from ginger oleoresin
with D4020 macroporous resin

WANG Yan, DU Ailing*, XUE Yanming, DU Aiqin

School of Chemistry and Chemical Engineering, Shandong University, Jinan 250061, China

Received:2011-01-22 Online:2011-06-16 Published:2011-01-22

摘要/Abstract

摘要：

为了获得一种高效、洁净的姜酚分离方法,选用乙醇-水体系为柱层析方法流动相,固定相为D4020大孔吸附树脂,从超临界二氧化碳提取的姜油树脂中分离姜酚。用紫外分光光度法对各段洗脱液进行跟踪检测,确定含姜酚的洗出段。用高效液相色谱法(high performance liquid chromatography,HPLC)和气相色谱质谱联用法(gas chromatography-mass spectrometer, GC-MS)对含姜酚的洗脱液进行检测。研究结果表明,在保证86.11％收率的条件下,姜酚的峰面积百分数为71.98％;经二次柱层析纯化后,姜酚的峰色谱面积百分数可达96.27％,收率为76.75％。该方法在分离纯度和收率上都获得了好的效果。

关键词: 姜酚, 大孔树脂, 高效液相色谱法, 气相色谱质谱联用法

Abstract:

In order to find an efficient clean method to separate the gingerol, a ethanolwater solution was used as mobile phase, and the D4020 macroporous resin was used as the stationary phase. Based on column chromatography analysis, the gingerols were separated from supercritical carbon dioxide extracted ginger oleoresin. Eluates in every stage were measured by UV to find out the sections containing gingerols. Then, the samples containing gingerols were measured by HPLC and GC-MS. The results showed that a average peak area percentage was 71.98％ at the yield of 86.11％. After further purification, the peak area percentage of gingerols rose to 96.27％ at a yield of 76.75％. This method could achieve good results in both purity and yield.

Key words: gingerols, macroporous resin; , high performance liquid chromatography(HPLC), gas chromatography-mass spectrometer (GC-MS)

王艳,杜爱玲*,薛岩明,杜爱琴. D4020大孔树脂分离姜油树脂中的姜酚[J]. 山东大学学报(工学版), 2011, 41(3): 148-153.

WANG Yan, DU Ailing*, XUE Yanming, DU Aiqin. Separation of gingerols from ginger oleoresin
with D4020 macroporous resin[J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2011, 41(3): 148-153.

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