Abstract: According to the variation of perchlorate mass concentration as a function of time in a series of batch tests, the influence on perchlorate removal by perchlorate reducing microbes (PRMs) associated with Fe0 as electron donor was investigated, which was caused by some relative factors, such as Fe0 adding amount, bacteria concentration, perchlorate load, and initial pH. The results showed that unlike some research before, the Fe0 adding amount was not as much as better. The optimum Fe0 adding amount (mass concentration) with perchlorate mass concentration at 10000 μg／L was about 40g／L. In this case, enough electrons could be produced, while the pH increased not too fast. The perchlorate load for PRMs used in this research was about 603μg／(L?hr?gPRMs), which could take the place of pure microbes to undertake perchlorate removal in the environmental water. The optimum pH for PRMs was around 7.0. The PRMs were more sensitive to the variation of initial pH, but they could survive in a wider range of pH during the reaction. In addition, the changes of the main PRMs species were analyzed through building phylogenetic tree. Compared with the microbes after incubation, the bacteria species partly changed after the experiments. This research may provide a theoretical support for the application of perchlorate removal by PRMs associated with Fe0 to a certain degree.

Key words: perchlorate, Fe0, perchlorate reducing microbes, Fe0 adding amount, pH