Abstract:

In order to find an efficient clean method to separate the gingerol, a  ethanolwater solution was used as mobile phase, and the D4020 macroporous resin was used as the stationary phase. Based on  column chromatography analysis, the gingerols were  separated from supercritical carbon dioxide extracted ginger oleoresin. Eluates in every stage were measured by UV to find out the sections  containing gingerols. Then, the samples containing gingerols were measured by HPLC and GC-MS. The results showed that a  average peak area percentage was 71.98％ at the yield of 86.11％. After further purification, the peak area percentage of gingerols rose  to 96.27％ at a  yield of 76.75％. This method could achieve good results in both purity and yield.

Key words:  gingerols, macroporous resin; , high performance liquid chromatography(HPLC), gas chromatography-mass spectrometer (GC-MS)