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山东大学学报(工学版) ›› 2016, Vol. 46 ›› Issue (3): 1-6.doi: 10.6040/j.issn.1672-3961.2.2015.044

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赤潮藻显微图像自动识别方法

乔小燕   

  1. 山东工商学院数学与信息科学学院, 山东 烟台 264005
  • 收稿日期:2015-06-23 出版日期:2016-06-30 发布日期:2015-06-23
  • 作者简介:乔小燕(1982— ),女,山东烟台人,副教授,博士,主要研究方向为图像处理与模式识别. E-mail:qiaoxy1026@126.com
  • 基金资助:
    国家自然科学基金资助项目(61401255);山东省优秀中青年科学家科研奖励基金资助项目(BS2012DX025);山东省社会科学规划资助项目(15DJJJ14)

Automatic recognition method of microscopic image of harmful algae

QIAO Xiaoyan   

  1. College of Mathematic and Information Science, Shandong Institute of Business and Technology, Yantai 264005, Shandong, China
  • Received:2015-06-23 Online:2016-06-30 Published:2015-06-23

摘要: 利用藻种细胞生物形态差异进行图像分析是浮游生物显微图像识别的一种有效方法,但存在藻种库单一、局部生理特征难以形式化描述等难题。为了克服以上难题,将赤潮藻显微图像识别分解为精确分割、特征提取、特征降维和分类诊断四个渐进识别过程。采用多方向投影积分的方法定位分割出细胞目标,进一步对顶刺和横沟细节区域实现了精细分割;将藻种生理特征与机器识别特征一一对应,依次对形状、不变矩、纹理和局部形态两级特征进行了有效地特征提取和描述;采用支持向量机多类别分类模型进行分类识别。研究结果表明,该方法能准确分割目标,可对不同角度拍摄的15类藻种细胞显微图像完成快速分类。

关键词: 横沟分割, 顶刺分割, 显微图像识别, 生物形态特征

Abstract: At present, the microscopic image identification of harmful algae based on biological morphological features are applied widely. However, there are some challenges, such as limited algae species resources, the difficulty of describing biological detailed features. To solve these problems, the identification of harmful algae was divided into four gradual processes: precise segmentation, feature extraction, feature dimension reduction and classification. First, the method based on projection and integral on multiple directions was proposed to extract cell object, and then the spine and cingulum were extracted. Second, the effective description were proposed after shape features, moment invariants, texture features and domain specific features were extracted. Then, SVM classifier was designed to recognize objects. Experimental results showed that this algorithm could achieve the quicker and better segmentation and recognition of 15 species of algae.

Key words: cingulum segmentation, microscopic image recognition, spine segmentation, biological morphological features

中图分类号: 

  • TP391
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