AB8大孔树脂吸附与硅胶层析法分离姜酚的对比研究

山东大学学报(工学版) ›› 2010, Vol. 40 ›› Issue (2): 121-125.

AB8大孔树脂吸附与硅胶层析法分离姜酚的对比研究

贾雁高，杜爱玲*,谷成燕, 杜爱琴,高宇

山东大学化学与化工学院, 山东济南 250100

收稿日期:2009-01-17 出版日期:2010-04-16 发布日期:2009-01-17
通讯作者: 杜爱玲(1956-),女,山东烟台人，教授，硕士，主要研究方向为天然产物有效成分的提取利用以及表面工程. E-mail:E-mail:duail@sina.com
作者简介:贾雁高(1982-),男,山东菏泽人，硕士研究生,主要研究方向为天然产物有效成分的分离.E-mail:jiayangao@yahoo.cn
基金资助:
山东省科技发展计划资助项目(2007GG30009010)

The comparison of the effects of gingerols separation by AB8 macroporous resin adsorption and silica gel column chromatography

JIA Yan-gao， DU Ai-ling*, GU Cheng-yan, DU Ai-qin, GAO Yu

School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China

Received:2009-01-17 Online:2010-04-16 Published:2009-01-17

摘要/Abstract

摘要：

采用硅胶柱层析和AB8大孔树脂吸脱附两种方法分离姜油树脂中的姜酚,采用气相色谱-质谱(gas chromatography-mass spectrometry, GC-MS)对分离样品进行表征,用峰面积归一化法对测试样品的气相色谱-质谱结果进行分析,确定了分离样品中各组分的相对峰面积百分比。以姜酚标准样品为参比,确定了姜油树脂所含姜酚在分离样品中的富集率。结果表明硅胶柱层析法的分离样品中姜酚集中在第3、4段,其中第4段含量最高,共鉴定出19种成分,姜酚峰面积百分数为14.34％,富集率为59.01％;采用AB8大孔树脂分离时姜酚集中在洗脱液前5号样,其中2号样含量较高,鉴定出11种成分,姜酚峰面积百分数为55.68％,富集率为70.88％。

关键词: 气相色谱-质谱法, 姜酚, 大孔吸附树脂, 硅胶层析法

Abstract:

Gingerols in ginger oleoresin were separated by AB8 macroporous adsorption resin and silica gel column chromatography. The samples were characterized by the gas chromatography-mass spectrometry(GC-MS) method.The peak area normalization method was used to determine the relative percentage of the components and the rate of accumulation of gingerol was determined by contrasting with gingerol standard samples. The results showed that gingerols was concentrated on the 3rd and the 4th parts. It identified 19 ingredients in the 4th part, which has the highest concentration of gingerols. The peak area percentage of gingerols was 14.34％ and the absolute rate of enrichment was 59.01％ with the former method, and the gingerols mostly focused on the former 5 eluate, of which only 11 elements was identified with high content in sample 2. The peak area percentage of gingerols was as high as 55.68% with the absolute rate of enrichment 70.88％ by the latter method.

Key words: gas chromatography-mass spectrometry method, gingerols, macroporous adsorption resin;silica gel adsorption chromatography

贾雁高，杜爱玲*,谷成燕, 杜爱琴,高宇. AB8大孔树脂吸附与硅胶层析法分离姜酚的对比研究[J]. 山东大学学报(工学版), 2010, 40(2): 121-125.

JIA Yan-gao， DU Ai-ling*, GU Cheng-yan, DU Ai-qin, GAO Yu. The comparison of the effects of gingerols separation by AB8 macroporous resin adsorption and silica gel column chromatography[J]. JOURNAL OF SHANDONG UNIVERSITY (ENGINEERING SCIENCE), 2010, 40(2): 121-125.

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